Composite

Part:BBa_K5246042:Design

Designed by: Edgaras Zaboras   Group: iGEM24_Vilnius-Lithuania   (2024-09-25)


C.Crescentus CB2/CB2A hfsH-hfsK-hfsL Part of the polysaccharide tetrad assembly system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 96
    Illegal XbaI site found at 856
    Illegal PstI site found at 945
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 96
    Illegal PstI site found at 945
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 96
    Illegal BglII site found at 859
    Illegal XhoI site found at 1674
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 96
    Illegal XbaI site found at 856
    Illegal PstI site found at 945
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 96
    Illegal XbaI site found at 856
    Illegal PstI site found at 945
    Illegal NgoMIV site found at 154
    Illegal NgoMIV site found at 178
    Illegal NgoMIV site found at 182
    Illegal NgoMIV site found at 255
    Illegal NgoMIV site found at 381
    Illegal NgoMIV site found at 542
    Illegal NgoMIV site found at 2223
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NA


Source

NA

References